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Preliminary Study on Culture of Chicken Embryonic Stem Cells by Buffalo Rat Liver Cells Conditioned Medium
Chun-Hua Meng, Chuan-Sheng Zhang1, Na-Na Yang2, Zi-Yu Wang3, Shao-Xian Cao, Li-Xin Du4*
Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 1Department of Animal Science, Hebei Normal University of Science and Technology, Changli 066600, China; 2Insititute of Life Science, Taish
Abstract: The culture system of chicken embryonic stem (ES) cells by buffalo rat liver cells conditioned medium (BRL-CM) was optimized. Stage X blastoderm cells of Shouguang chicken were cocultured with feeder layers (CEF， MEF and STO) in BRL-CM. The clone was identified by morphology， PAS staining， AKP staining， constructing chimeras and differentiation in vitro. Results showed that BRL-CM could stimulate chicken ES cells proliferation. Feeder layers could maintain the proliferation and undifferentiation of chicken ES colonies. Compared with CEF feeder layers， MEF and STO feeder layers were more ideal in culture of chicken ES cells (P<0.05). The passage method of picking colonies was more efficient than trypsin digestion (P<0.05).
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