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Identification of Tumor Stem-like Subpopulation Cells in Human Leukemia Cell Lines KG-1a
Song Yang, Xiao-Ming Zhong, Ling Zhang*, Zhan-Peng Luo, Peng He, Yu-Jie Gao
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Key Laboratory, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
Abstract: To identify tumor stem-like subpopulation cells in human leukemia cell lines KG-1a， we observed the morphology and RNA content of KG-1a cells respectively using Wright's staining and acridine orange staining， then detected the cell cycle distribution and the CD34+CD38- subpopulation cells of KG-1a cells with flow cytometry， and also determined the expression of CD34 with immunohistochemistry assay and flow cytometry， at last detected the side population-like cells in KG-la cells using fluorescent microscope after Hoechst33342 staining. It was shown that KG-1a cells were primitive and nucleoli were apparent; RNA content in small subpopulation cells was low， and the G0/G1 cells were 15.5% about KG-1a cells. CD34 were generally expressed in cytoplasm and on membrane of KG-1a cells， the percentage of CD34+ cells was 96.3%， and the proportion of CD34+CD38- cells was 7.02%; the percentage of side population-like cells in KG-1a cells was 7.60%. Our study suggested that tumor stem-like subpopulation cells existed in human leukemia cell lines KG-1a.
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