Expression of RhoA in the Epithelial Ovarian Cells and the Correlation of RhoA with Ovarian Cancer Metastasis

Abstract: Two ovarian cancer cell lines， HO8910PM and HO8910， and one normal epithelial ovarian cell line HOSEA was cultured to investigate the expression of RhoA in the epithelial ovarian cells and its correlation with invasion and metastasis of epithelial ovarian carcinoma. Expression of RhoA in three cell lines was detected by immunohistochemical method. Matrigel invasion assay using three epithelial ovarian cell lines was done in Transwell chambers， so was angiogenic ability. Results demonstrated that the expression levels of RhoA varied in three cell lines examined. The expression levels of RhoA in HO8910 decreased comparing with HO8910PM but increased comparing with HOSEA (F=35.73， P<0.01). HO8910PM had the strongest invasiveness， HO8910 was sub-invasive， while HOSEA had non-invasive property (F=105.80， P<0.01). Angiogenic capability of HO8910PM was strongest， HO8910 was weaker， while HOSEA had no angiogenesis. The difference between angiogenic capability of three cells was statistically significant (F=24.69， P<0.01). The expression levels of RhoA in ovarian cancer cells was significantly positive correlated with both the invasiveness and angiogenic capability of these cells (r=0.89 or 0.84， P<0.05; r=0.81 or 0.88， P<0.05). These results suggest that expression of RhoA is correlated with both the invasiveness and angiogenic capability of the epithelial ovarian carcinoma cells in vitro. With the overexpression of RhoA， both invasiveness and angiogenesis of the epithelial ovarian carcinoma cells increase. RhoA may serve as an important parameter in the metastasis of epithelial ovarian carcinoma.
    

CSTR: 32200.14.cjcb.2008.04.0022