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Afr1p Regulates the Localization of Septin Cdc12p in Saccharomyces cerevisiae
Zhu Changwei1,2*, Xiong Mingyong2
1College of Life Science, Anhui Science and Technology University, Fengyang 233100, China; 2Department of Biochemical Engineering, School of Chemical Engineering and Technology, TianJin University, Tianjing 300072, China
Abstract: AFR1 is firstly identified to promote α-factor resistance when overexpressed. It also has an important role in promoting shmoo formation during mating. Afr1p regulates the localization and activity of the Mpk1p MAP kinase in the cell wall integrity pathway. Here， we showed that galactose-induced overexpression of AFR1 might interrupte the proper localization of Cdc12p， and deletion of AFR1 also cause mislocalization of Cdc12p in budding cells. The result of Western blot indicated that the expression of Afr1p was at a constant level during vegetative growth. These results suggest that AFR1 is required for the localization of septin Cdc12p and regulates the septin architecture in Saccharomyces cerevisiae.
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