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Biological Characteristics of Porcine Primordial Germ Cells
Ma Jing#, Zhang Yu#, Wang Jianyu, Xue Binghua, Wei Renyue, Li Yan, Liu Zhonghua*
College of Life Science, Northeast Agricultural University, Harbin 150030, China
Abstract: Embryonic germ cells (EGCs) are a kind of pluripotent stem cells derived from primordial germ cells (PGCs). Studies on biological characteristics of PGCs are favorable for the establishment of EGCs and understanding the development program of germ cells. In this study， we observed growth behaviors of primary porcine PGCs as well as expression patterns of pluripotency and germline markers during their reprogramming. The result showed that PGCs isolated from genital ridge of porcine embryos age of 26 d were alkaline phosphatase-positive cells， with larger size and nuclear-cytoplasmic ratio; the PGCs exhibited enhanced migration and proliferation abilities at the beginning of ex vivo cultivation， and the propagation reached a plateau at day 5， when the clones were with elevated levels of Oct4， Nanog， Sox2， c-Myc， Klf4 and Ifitm3 (P<0.05) and depressed level of Blimp1 (P<0.05)， compared with porcine embryonic fibroblasts.Besides， the primary clones had the potential to differentiate into different cell types.
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