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Effects of Montmorillonite on the Intestinal Barrier Function and Tight Junction Protein of Caco-2 Cells in Response to Escherichia coli K88
Cai-Hong Hu*, Hai-Ping Liu, Xiang-Dong Hu1, Yong Xu, Mei-Sheng Xia2
College of Animal Science; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310029, China; 1Zhejiang Crown Technology Co., Ltd, Hangzhou 310012, China; 2Department of Earth Science, Zhejiang
Abstract: Caco-2 cell line was used as an in vitro model to investigate the effects of montmorillonite (MMT) on the intestinal barrier function and tight junction protein of Caco-2 cells in response to E.coli K88. The transepithelial electrical resistance (TEER) and permeability of the paracellular transport marker mannitol were measured to evaluate the intestinal barrier function. Changes in subcellular localization of the tight junction protein Occludin protein by Immunohistochemistry were also assessed after treatment of MMT. The results showed that the TEER and the transmissivity of mannitol increased after infected by E.coli K88， and the increase was decreased after dealing with MMT (P<0.05). The TEER of Caco-2 cell infected by E.coli K88 was increased with the concentration of MMT at the concentration range of 0-1 g/L and remained steady at the concentration range of 1-1.67 g/L. The structure of tight junction was destroyed and the expression of Occludin was diminished after infected with E.coli K88. But MMT can prevent the damage induced by E.coli K88. The results indicated that MMT exerts a protective effect against the damage to integrity of Caco-2 monolayer cells and the tight junction structure and distribution of tight junction proteins induced by E.coli K88 infection.
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