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The Expression Pattern of Methyl-CpG Binding Domain Protein 2 (MBD2) in Mouse Endometrium during Preimplantation
Zhou Yiqing, He Junling, Chen Xuemei, Wang Yingxiong, Liu Xueqing, Ding Yubin*
Laboratory of the Reproductive Biology, School of Public Health and Management,Chongqing Medical University, Chongqing 400016, China
Abstract: The experiment was aim to explore the expression pattern of Methyl-CpG binding domain protein 2 (MBD2) during peri-implantation in the mouse endometrium. Expression of MBD2 mRNA and MBD2 protein in the non-pregnant and pregnant mouse endometrium from d0 to d7 was examined by Real-time fluorescence quantitative PCR (qPCR)， Western blot and immunohistochemistry. MBD2 mRNA expressed in uterine endometrium of the non-pregnant (d0) and pregnant mouse (d1 to d7). MBD2 mRNA increased gradually from d5 to d7. Western blot analysis showed that the protein expression of MBD2 in mouse endometrium had the similar expression pattern to its mRNA that detected by Real-time fluorescence quantitative PCR. Moreover， from pregnant d1 to d4， MBD2 was expressed in epithelium， glandular cells and stromal cells in the endometrium. On d5 to d7， MBD2 was more intensely expressed in stromal cells， especially in the decidual zone. The expression of MBD2 in the pseudopregnant on d1 and d4 was expressed in epithelium， glandular cells and stromal cells in the endometrium.But it was significantly lower in the stromal on d5 to d7. Using artificial decidualization and treatment with antisense oli-godexynucleotides of MBD2， the expression of MBD2 in the uterus was remarkably inhibited and the level of decidualization was decreased. These results suggested that MBD2 was involved in modulating the decidulization of mouse uterine.
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