Home > Browse Issues > Vol.36 No.7
Vitrification of Oocytes and Embryos of Macaca fascicularis and Macaca mulatta
Huang Zhangqiong1, Xue Zhigang2, Gao Jiahong1, Jiang Qinfang1, Wu Zhengcun1,Li Cong1, Han Yuanyuan1, Zeng Qiao2*, Ma Kaili1*
1Center for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences,Kunming 650118, China; 2Research Center for Translational Medicine, Shanghai Tongji Hospital,Medical School of Tongji University, Shanghai 200065, China
Abstract: In order to retrench funds and preservation of variety resources of transgenic animal model， this study adopted vitrification method to cryopreserve and thaw 183 oocytes (at the GV， MI and MII stages)， 144 embryos in cleavage stage (at the 2- to 8-cell stages) and 25 mulberry of the nonhuman primate with the solution prepared by ourselves. Including 67 oocytes， 45 embryos in cleavage stage and 11 mulberry of cynomolgus monkey; Including 116 oocytes， 69 embryos in cleavage stage and 14 mulberry of rhesus monkey. After thawing， the survival rate was respectively 83.58% (56/67)， 80% (36/45)， 81.82% (9/11)， 87.93% (102/116)， 79.71% (55/69) and 78.57% (11/14). The results showed that vitrification cryopreservation， simple and effective， is a good method to cryopreserve the oocytes and embryos of rhesus monkey and cynomolgus monkey.
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