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The Screening and Identification of Androgen Receptor Target Genes in Androgen-independent Prostate Cancer Cell
Xu Siqi1,2, Liao Zhaoping1,2, Liu Chunhua3,2, Cheng Yue2, Ji Lili1,2, Duan Xiuzhi2, Chen Yuhua2, Tao Zhihua1,2*
1Department of Clinical Laboratory Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; 2Department of Laboratory Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009,China; 3School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325000, China)
Abstract: Chromatin immunoprecipitation assay was performed to screen the androgen receptor binding sites in androgen-independent prostate cancer cell LNCaP-AI in the whole genomic. Using high-throughput sequencing and bioinformatic analysis， there were 2 876 peaks (p-value<1×10–5)， and the average length of peaks was 673 bp; locating each peak in the Hg19 genome， 1 865 genes were founded. There were 425 genes which the fold enrichment was more than 10. It could be founded that the top five genes were associated with cell， cell part， cellular process， binding and organelle by GO analysis of peak related genes. Genes associated with focal adhesion， metabolic pathways， transcriptional misregulation in cancer and purine metabolism were the majority by pathway analysis of peak related genes. Seven candidated AR target genes were selected to analyze the reactivity to DHT stimulation in LNCaP-AI cell and LNCaP cell by Real-time qPCR. The result showed that DHT stimulation could change the expression of seven candidated target genes in LNCaP-AI cell. Our data play a vital role in the further study on androgen receptor and it’s regulated target genes in the process of androgen-dependent prostate cancer progress to androgen-independent prostate cancer.
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