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Preliminary Study on the Expression Level of Xinjiang Strain HPV-16 L1 in Eukaryotic Cells
Lu Xu, Li-Hong Liu, Yi-Jie Li, Fu-Chun Zhang, Zheng-Hai Ma*
College of Life Science and Technology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Xinjiang University, Urumqi 830046, China
Abstract: To contrast the expression level of Xinjiang strain HPV-16 L1 and codon optimizing HPV-16 L1 in eukaryotic cells. Xinjiang strain HPV-16 L1 and codon optimizing HPV-16 L1 were cloned into pcDNA3.1 respectively. The recombinant plasmids were infused quickly into the mouse through tail vein or transfected into 293T cells， and then the transcripts of HPV-16 L1 were detected in the liver of the mouse and in 293T cells with RT-PCR， HPV-16 L1 protein was detected in 293T cells with Western blotting. The results show that Xinjiang strain HPV-16 L1 and codon optimizing HPV-16 L1 can be transcribed in the liver of the mouse and in 293T cells， and the L1 protein expression levels of Xinjiang strain HPV-16 L1 is lower than that of codon optimizing HPV-16 L1 markedly.
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