Home > Browse Issues > Vol.36 No.7
Effect of Growth Differentiation Factor-9 (GDF-9) on Bovine Cumulus Cells Proliferation
OuYang Xiaoqing, Zhang Chunqiang, Li Ruilan, Zhang Tong, Fan Xiaomei, Gao Lixia, Zhao Meng, Zhang Jiaxin*
Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract: This paper investigated the effect of growth differentiation factor-9 (GDF-9) on the proliferation of bovine cumulus cells. The effect of GDF-9 with different concentration on cumulus cells proliferation was studied by MTT assay. MTT results showed that GDF-9 could promote cumulus cell proliferation by a concentration-dependent manner， and FSH had a synergistic effect with GDF-9 on inducing cumulus cell proliferation. In addition， GDF-9 combined with FSH could promote oocytectomized cumulus complexes (OOX) development. The expression of cumulus cell expansion-related genes PTX3， HAS2 and PTGS2 were examined by Real-time quantitative PCR. The results showed that the expression of expansion-related gene were up-regulated by GDF-9. In conclusion， GDF-9 can promote cumulus cells proliferation and play an important role in regulating function of cumulus cells.
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