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Low Expression of USP9X Promoted Apoptosis of Hepatoma Cells Through Mcl-1 Down-regulation
Hu Huiwen1, Tang Chengyong2, Jiang Qinghu1, Luo Wei1, Liu Jiming1, Wei Xufu1, Liu Rui1, Wu Zhongjun1*
1Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2Department of Clinical Pharmacology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract: In this paper， we investigated the regulation of inhibiting ubiquitin-specific protease 9X (USP9X) on myeloid cell leukemia-1 (Mcl-1) protein expression and its effect on apoptosis and viability of human hepatocellular carcinoma (HCC) cells SMMC7721 and HepG2. There were two groups in the study， USP9X-siRNA group and NC group. The protein expression of USP9X was detected in SMMC7721， HepG2 and normal human liver cell line L02. SMMC7721 and HepG2 cells were infected with USP9X-siRNA， and cell apoptosis and cell growth viability were analyzed by flow cytometry and MTT. Mcl-1， a potential target of USP9X， was detected by Western blot. We found that the protein expressions of USP9X in SMMC7721 and HepG2 were both higher than that in L02 (t=15.155， P=0.000; t=9.171， P=0.001). Inhibiting expression of USP9X in SMMC7721 and HepG2 cells obviously suppressed Mcl-1 protein expression as well as increased cell apoptosis and decreased cell viability. These results suggested that expression of USP9X was upregulated in hepatoma cells SMMC7721 and HepG2， and inhibiting USP9X might induce cell apoptosis in hepatocellular carcinoma cells SMMC7721 and HepG2 by down-regulating Mcl-1 protein expression.
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