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Function and Expression Pattern Investigation of Gas6 on Porcine Oocytes and Early Embryos
Li Zhipeng, Lei Xiaocan, Lu Xingrong, Zhu Peng, Cui Kuiqing, Shi Deshun*, Liu Qingyou*
State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China
Abstract: The expression pattern of Gas6 gene in porcine oocytes and early embryo was analyzed systematically using immunohistochemistry and QRT-PCR， and a new method was proposed to improve the quality of in vitro matured pig oocytes. The results showed that Gas6 gene was localized in ovarian oocyte nucleus and the surrounding cumulus cells simultaneously. It expressed all through the in vitro maturation of oocytes， and reached the highest relative expression peak in the blastocyst stage. Gas6 mRNA existed in the process of in vitro matured oocytes continuously， but disappeared after parthenogenetic activation， reappeared until the blastocyst stage. By adding recombinant Gas6 protein into the in vitro matured media of pig oocytes， we found that there was no significant change to the poly body discharge rate of the in vitro matured oocytes， but the cleavage rate， blastocyst rate and blastocyst cells number of oocytes after parthenogenetic activation was significantly increased when the adding concentration reached 100 ng/mL. We predicted that Gas6 increased the development potential of oocytes by improving the quality of oocytes cytoplasm maturation， so as to obtain more and better embryos.
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