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Expression of Human LIPIN1 Gene and Its Functional Analysis in Yeast
Fang Zhijia1, Du Xiuxiu1, Huang Zhiwei1*, Wang Song2, Shi Ping2*
1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; 2State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Abstract: The regulation of lipid metabolism is important in maintaining the energy balance. Its disorder induces many diseases， such as obesity， diabetes， high blood pressure and so on. LIPIN1， a key gene for adipocyte differentiation induction and lipids synthesis regulation， encodes phospholipids phosphatase (PAP) and plays an important role in triglyceride synthesis and lipids homeostasis in human. In addition， LIPIN1 acts as a transcriptional coactivator in various regulations of nutrition and growth metabolism， and many similar genes have been found in other species， indicating its various functions and species conservation. In this paper， we constructed PAH1 mutant yeast and transformed it with human LIPIN1 to study effects of LIPIN1 on yeast growth and lipid synthesis based on the convenience of Saccharomyces cerevisiae in lipid metabolism study and phenotypic observation， and the clearity of cellular function of PAH1. Thereafter， we compared LIPIN1p with PAH1p in structure and function via functional complementation analysis and bioinformatics， and confirmed the functional conservation of PAP in human and yeast. This work will provide evidence for the cellular function study of LIPIN1.
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