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Effect of Lipoxin A4 on MicroRNAs Expression Profile in HepG2 Cells through Modulating the Tumor Associated Macrophages
Huang Wanqiu1#, Ma Mingyang2#, Cheng Xue3, Wang Chulin3, Miao Shuo3, Wu Ping3*
1Eight-year Program of Clinical Medicine, 2010 Session, Basic Department of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2Hepatic Surgery Center, Tongji Hospital, Huazhong University of
Abstract: On the basis that the pathologic process of tumor is closely related to uncontrolled inflammation， we have proved that Lipoxin A4 (LXA4) could inhibit the proliferation and metastasis of HCC cells both in vitro and in vivo. This study further investigated the indirect effect of Lipoxin A4 (LXA4) on the microRNA (miRNA) expression profile in HepG2 cells through modulating the tumor-associated macrophages. Conditioned cell culture media from LPS-stimulated and LPS/LXA4 co-stimulated U937 cells， as ACM and LCM respectively， were collected. HepG2 cells were separated into control group， ACM group and LCM group， which were cultured in normal culture media， ACM and LCM for 24 hours， respectively. miRNAs were extracted and hybridized to miR-CURY™ LNA Array (V16.0). It was considered to be up- or down- regulated when the miRNAs fluorescent intensity ration between two groups was over 2 or less than 0.5. Validation of microarray results was carried out by Real-time PCR of hsa-miR-623. Compared with control group， ACM treatment for 24 h up-regulated 35 miRNAs and down-regulated 130 miRNAs in HepG2 cells， while LCM group had 185 miRNAs higher and 71 miRNAs lower than ACM group. Hsa-miR-623 showed similar variation with microarray. Our study indicated that LXA4 could indirectly regulate the miRNAs expression profile in HepG2 cells through modulating the tumor associated macrophages.
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