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The Effects of Ska2 Silencing on Cellular Proliferation， Migration and Invasion of H1299 Lung Cancer Cells
Wang Yitao1,2, Cai Wei1,2, Zhu Yuanyuan1,2, Li Xiyue1,2, Zhang Chundong1,2, Wang Sen1,2,Lei Yunlong1,2, Zhang Ying1,2, Zhu Huifang1,2, Li Yi1,2
1Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China; 2Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China
Abstract: Ska2 (spindle and kinetochore associated complex subunit 2)， also known as FAM33A (family with sequence similarity 33， member A)， is a recently identified gene involved in both cell cycle regulation and tumorigenesis， and it shares a bidirectional promoter with PRR11 (proline rich 11). In the present study， we utilized lung cancer cell line H1299 to construct Ska2-slienced cell strain by RNAi， and analyzed the cellular phenotype and potential molecular mechnism. The results of RT-PCR and Western blot revealed that Ska2 was efficiently silenced at both mRNA and protein levels. Phenotypic analysis revealed that the proliferation， migration and invasion activities were significantly inhibited in Ska2-silenced stable cell strain compared with those of the control cells. In addition， silencing of Ska2 resulted in the downregulation of CCNA1. Taken together， our results strongly suggested that Ska2 and its neighboring gene PRR11 had similar functions and might play important roles in regulating the proliferation， migration and invasion of lung cancer cells.
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