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Influence of Exhaustive Exercise Induced Oxidative Stress on Band 3 of Rat Erythrocyte
Xiong Yanlian, Xiong Yanlei, Li Yaojin, Tang Fuzhou, Zhao Yajin, Wang Xiang*
Key Laboratory of Biorheology and Technology, Chongqing University College of Bioengineering, Chongqing 400044, China
Abstract: The aim of the present study was to explore the effect of exhaustive exercise-induced oxidative stress on the Band 3 of rat erythrocytes. Rats were divided into sedentary control (C) and exercise test (ET) groups. The ET group was divided further into exhaustive running exercise (ERE) and moderate running exercise (MRE) subgroups. Oxidative stress-induced erythrocyte injure and antioxidant capacity were analyzed. Erythrocytes from control and running exercise groups were evaluated for the expression of erythrocyte Band 3 through immunoblot and immunofluorescence studies. Besides， the anion-transport activity was investigated. The results showed that red blood cells were subjected to severe oxidative stress under exhaustive exercise conditions. Lipid peroxidation and protein sulfhydryl cross-link based clustering of membrane was found after exhaustive exercise， and exhaustive exercise also led to significant increments in the levels of clustering of erythrocyte Band 3 and the decrease of anion-transport activity. Our results suggested that exhaustive running exercise resulted in elevated oxidative stress in erythrocytes， and this change could be associated with the alterations in the structure and function of erythrocyte Band 3.
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