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Preliminary Studies on Biological Characteristics of Rhesus Monkey (Macaca mulatta) Dendritic Cells
Chang-Yong Ge, Hong-Jun Li1, Tian-Hong Xie1, Guang-Ming Zhang1, Shan Yi1, Mao-Sheng Sun, Nan-Ping Wu*
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China; 1Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking U
Abstract: Dendritic cell (DC) play a crucial role both in the primary HIV infection stage and in the latent HIV infection period. The rhesus macaque， whose biological characteristics are remarkably similar to those of humans， is a good animal model to study the AIDS pathogenesis. The biological characteristics of the rhesus monkey dendritic cells need to be known before the immune function of rhesus monkey dendritic cells in HIV infection will be studied. Rhesus monkey (Macaca mulatta) monocytes were separated from peripheral blood. Monocytes were proliferated and induced to differentiate toward DC by using recombinant human granulocyte- rhGM-CSF， rhIL-4， and impulsed by using inactive SV40 antigen， and promoted for mature DC by using rhTNF-α The morphocytology of DC was observed under ordinary light microscope and scanning electron microscope. The characteristic dendritic morphology was observed on some DC surface. High expression of HLA-DR and CD86 on rhesus monkey DC were mensurated. Rhesus monkey DC derived from inducement in vitro can cause auto T cell proliferation effectively in mixed lymphocyte reaction (MLR). At day 9 of cell culture， flow cytometric analysis showed that cells with CD1a account for 60.5%±2.0%， and cells with CD83 account for 43.8%±1.5%. DC endocytosis of horseradish peroxidase (HRP) showed that the peak value of endocytic capacity of DC lies at the sixth day of cell culture. This study laid the foundation for the research and development of DC by using rhesus monkey as animal model.
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