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Effects of Glucose Level and BMP4 on Osteogenic Differentiation of Mouse Induced Pluripotent Stem Cells
Xing Jianyu1#, Zhang Yu2#, Chen Mo2, Wang Yibo1, Ding Chao1, Shi Jiuhui1*, Liu Zhonghua2*
1The First Affiliated Hospital of Harbin Medical University, Harbin 150006, China; 2College of Life Science, Northeast Agricultural University, Harbin 150030, China
Abstract: Induced pluripotent stem (iPS) cell technology casts a light on autologous cell/tissue transplantation. Considering that diabetes is combined with delayed bone regeneration， so in this study， we investigated osteogenic capability of mouse iPS cells in the media with different glucose levels and evaluated effect of BMP4 (bone morphogenetic protein 4) on the osteogenic capability of mouse iPS cells. The results showed that， after 21 days of osteogenic induction， low-glucose-level group had a higher percentage of alizarin red S-positive cells and elevated mRNA levels of osteogenic genes， Runx2 and Osteocalcin， compared with those of the high-glucose-level group and spontaneous differentiation group (P<0.05); BMP4 treatment increased the percentage of alizarin red S-positive cells and expression level of Osteocalcin in high-glucose-level group (P<0.05)， but had no effect in spontaneous differentiation group. The results indicated that low glucose level promoted osteogenetic differentiation of mouse iPS cells; Although BMP4 could enhance osteogenic ability in high-glucose-level group， it functioned specifically during osteogenesis.
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