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Cloning and Physical Mapping of rRNA on Metaphase Chromosomes in Sunflower (Helianthus annuus L.)
Gao Meng1,2, An Yulin1*, Sun Ruifen1, Zhang Yanfang1
1Biotechnology Research Center, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot 010031, China; 2College of Agronomy, Inner Mongolia Agriculture University, Huhhot 010019, China
Abstract: In this paper， 5S rRNA and 18S rRNA gene fragments were cloned from the triple hybrid of “Inner Mongolia Hibrid oil sunflower 3” and their sequences were analysed. The lengths of 5S rRNA and 18S rRNA gene fragments were 515 bp and 1 808 bp， respectively. In fluorescence in situ hybridization， the probes for 5S rRNA， 18S rRNA and 45S rRNA were hybridized with sunflower genomic DNA from the triple hybrid of “Inner Mongolia Hibrid oil sunflower 3”. Three pairs of hybridization signals were detected at the same loci both with 45S rRNA and 18S rRNA probes: at the end of shot arms of chromosome pairs 3， 10 and satellite chromosome pairs 2. 45S rRNA probe showed the same intensity of hybridization signals. 5S rRNA showed two pairs signals at the end of the short arms of chromosome pairs 7 and 10.
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