Effects and Possible Mechanisms of BMP9 on the Invasion and Migration of Human Lung Cancer A549 Cells

Abstract: Bone morphogenetic protein 9 (BMP9)， which belongs to the transforming growth factor-β superfamily，regulates a wide range of cellular responses including cell proliferation， differentiation， adhesion， migration， and apoptosis. Human lung cancer A549 cells were used as experiment cells and the over-expression of BMP9 was infected by adenovirus in vitro. RT-PCR and Western blot were used to detect the expression of BMP9 in recombinantAdBMP9 cells; To detect the changes of the recombinant AdBMP9 cells， invasion and migration， cell wounding assay and Transwell invasion assay were carried out. At last， RT-PCR and Western blot were used to detect the expre-ssion of IL-6， and Western blot was used to detect the expression of total Akt and phosphorylation Akt after BMP9 adenovirus infection. These results showed that BMP9 mRNA and protein were expressed in recombinant AdBMP9 cells; The healing rate of experimental group was increased from (85.4±2.1)% and (86.5±3.4)% in control groups to (97.4±2.6)% (P<0.05)， and the number of invasion cells which moved across the matrix barrier in experimental group was increased from (105.5±13.1) and (113.3±14.9) in control groups to (224.3±24.6) (P<0.05). In comparison with the control groups， the expression of IL-6 and phosphorylated Akt were significantly increased in AdBMP9 group. We can draw the conclusion that BMP9 can promote the invasion and migration of lung cancer A549 cells possibly through up-regulation of IL-6 and activation of the PI3k/Akt cell signaling pathway.

CSTR: 32200.14.cjcb.2014.02.0004