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The Inhibitory Effect of RNAi Shp2 Gene on the Proliferation of Chronic Myeloid Leukemia K562 Cells
Wang Canwei1, Tao Kun1*, Qi Jieyu1, Deng Yiping2
1Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China; 2Chongqing Medical University Experiment Center, Chongqing 400016, China
Abstract: We established low-expressed Shp2 recombinant plasmids containing siRNA fragment specific for Shp2 gene. Real-time PCR and Western blot assays were used to detect the mRNA and protein level of bcr/abl gene. The proliferation of cells was detected by MTT assay， and the apoptosis was detected by Flow Cytometry (FCM). The results demonstrated that the recombinant plasmids were successfully constructed and significantly inhibited the expression of Shp2 gene. Compared with the two control groups， the mRNA and protein level of bcr/abl were significantly decreased; the proliferation ability was inhibited significantly (P<0.05) and the rate of apoptosis cells was increased significantly (P<0.05). The biological effect of K562 cells may be significantly inhibited by down-regulation of Shp2， indicating that Shp2 may play a biological role as a tumor suppressor gene.
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