Effect of LATS1 Over-Expression on Proliferation and Cell Cycle Regulation of A549 Cells

Abstract: By over-expressing large tumor suppressor gene 1 (LATS1)， we aimed to investigate its effect on the proliferation and cell cycle regulation in lung cancer cell A549. LATS1 gene coding region was cloned into lentivirus vector， and then lentivirus particles were transfected into A549 cells to up-regulate the expression of LATS1 gene. The expression of LATS1 and YAP were detected at both mRNA and protein levels by RT-PCR and Western blot. The apoptosis and cycle of cells were detected by flow cytometry. The proliferation level of cells was detected by CCK-8. The expression levels of LATS1 mRNA and protein were up-regulated significantly after the lentivirus vector of LATS1 over expression transfected into A549 cells. Meanwhile， the expression levels of YAP mRNA and protein were down-regulated. 5th days after transfection with lentivirus vector of LATS1 over expression， the proliferation rate of transfected cells was lower than untransfected cells (P<0.05). Flow cytometry showed that more cells of transfected went into phase G1 and fewer cells went into phase S. Moreover， apoptosis rate was significant increased in transfected cells (P<0.05). The results demonstrate that LATS1 can promote A549 cells apoptosis， induce G1 phase arrest and inhibit the cell proliferation by down-regulating the expression of YAP.

CSTR: 32200.14.cjcb.2014.01.0009