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The Detoxification Effect of Overexpressed Shark SBP1 on HL-7702 Cells under Cadmium Stress
Wang Yong1*, He Manwen2, Sun Yanmei2, Liu Qiong1
1College of Life Science, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University,Shenzhen 518060, China; 2College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering,Shenzhen University,
Abstract: Selenium-binding protein 1 (SBP1) is involved in tumorigenesis and has the ability to bind the heavy metal cadmium. In this study， recombinant plasmid of white tip shark SBP1 was constructed and transfected into HL-7702 cells. CCK-8 and CFDA SE were used to detect cell proliferation under Cd stress; flow cytometry was used to analyze intracellular ROS levels. Under 100 μmol/L of CdCl2 stress， the cell viability of expression SBP1 group was 76.4%， which was approximately 2-fold of the control group; meanwhile， the cell proliferation index of experimental group was also higher than that of control group. Furthermore， we speculated that the reason for shark SBP1 expression efficiently helps to detoxify Cd for HL-7702 cells was based on the 2 CXXC， 2 HXD and 3 HXXH motifs of it， which is speculated antioxidation and metal binding sites respectively. In addition， a significantly low intracellular ROS levels was detected for HL-7702 cells after shark SBP1 was expressed， which suggested that reducing oxidative stress induced by heavy metal may also play a role for the detoxification of Cd.
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