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Effects of Scinderin Silence on the Proliferation and Metastasis of Human Gastric Cancer Cell SGC-7901
Chen Xiaomin1, Le Donghai2*, Li Keqiang1,2*, Guo Junming1, Chen Ping2, Mao Liangang2, Feng Weiyun2
1Medical School of Ningbo University, Ningbo 315211, China; 2Ningbo No.2 Hospital, Ningbo 315010, China
Abstract: Scinderin is a Ca2+-dependent filamentous actin (F-actin) severing and capping protein， which plays a key role in secretion. But few researches about biological effects of scinderin on human disease especially neoplasm are currently reported. In this study， we transfected the shRNA targeting scinderin lentiviral vector into human gastric cancer cell line SGC-7901. The efficiency of transfection was observed under fluorescence microscope. Effects of gene silencing were confirmed by RT-qPCR and Western blot. The proliferation was analyzed by real-time cell analyzer (RTCA) and the cell cycle distribution was investigated by flow cytometer. The migration was analyzed by Transwell. Results showed that scinderin-shRNA was transfected into SGC-7901 successfully. After transfection， levels of scinderin mRNA and protein expression reduced significantly (P<0.01). The abilities of cell proliferation and migration decreased obviously (P<0.05)，while cell cycle was arrested in the G2/M phase. Consequently， we can draw the conclusion that silencing scinderin can effectively restrain the proliferation and metastasis of human gastric cancer cell SGC-7901， which will be an experiment support for the possible regulation of scinderin in gastric cancer evolvement.
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