The Evaluation of Anti-angiogenic Treatment for Implanted Rabbit VX2 Breast Tumor Using Multi-slice Spiral Computed Tomography

Abstract: The objective of this work was to investigate the correlation between multi-slice spiral computed tomography (MSCT) perfusion imaging and VEGF for assessing targeted anti-angiogenesis therapy on an implanted rabbit VX2 breast tumor model. 69 female rabbits were randomly assigned to one of the 4 groups and received treatment accordingly: control (saline)， Endostar， neoadjuvant chemotherapy (CEF) and combination therapy (Endostar and CEF). After 2 weeks of treatment， MSCT perfusion scannings were performed for all rabbits and information about blood flow (BF)， blood volume (BV)， mean transit time (MTT) and surface permeability (PS) was collected. Then tumor tissue was dissected for immunohistochemistry and Western blot for VEGF expression.There was a positive correlation between VEGF expression and BE， BV， or PS level (P<0.05) and a negative correlation between VEGF expression and MTT level for all 4 groups (P<0.05). Therefore， MSCT can be used as a non-invasive approach to evaluate the effect of anti-angiogenic therapy for implanted rabbit VX2 breast tumors.

CSTR: 32200.14.cjcb.2014.01.0003