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The Effects of Ovarian Cancer Cells Co-cultured with Macrophages on the Expression of B7-H1 and Its Mechanisms
Xiong Haiyu, Ma Tingting, Wang Qin, Dong Jinyu, Liang Qindong, Li Ziwei, Zhang Weili, Tu Zhiguang*
College of Laboratory Medicine, Key Laboratory of Laboratory Medical Diagnostics of Education Ministry,Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the effects of ovarian cancer cells co-cultured with macrophages on the expre-ssion of B7-H1 and its possible mechanisms， phorbol 12-myristate 13-acetate (PMA) treated THP-1 cells or human monocytes were co-cultured with human ovarian cancer cell line SKOV3 for 24 h in vitro without direct contact， the expression of B7-H1 in SKOV3 cells and macrophages was detected by qRT-PCR， Western blot and flow cytometry， respectively; In addition， the expression of B7-H1 was also determined after treating the coculture system with inhibitors of NF-κB， JAK2/STAT3 or p38 MAPK signal pathways. These results suggested that the expression of B7-H1 was significantly elevated at both mRNA and protein levels in SKOV3 and macrophages after coculture for 24 h (P<0.05). However， the upregulation of B7-H1 expression was inhibited by blocking NF-κB， JAK2/STAT3 or p38 MAPK signal pathways (P<0.05). SKOV3 cells co-cultured with macrophages promoted the expression of B7-H1 (P<0.05)， which was possible involved in the activation of NF-κB， JAK2/STAT3， p38 MAPK signal pathways.
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