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Isolation and Culture of Fibroblast Cells from Bos mutus and Analysis on Some Biological Characteristics
Guo Zhilin1,2*, Yang Yongmei3, Zhao Mingde1, Yu Jun1, Wang Wei1, Pang Bo2, Guo Songchang2, Zhao Xinquan2
1Academy of Chemistry and Life Science, Qinghai University for Nationalities, Xining 810007, China; 2Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining 810008, China; 3Academy of Public Management,
Abstract: Bos mutus is the rare bovine species under first-grade protection and is native to the Qinghai-Tibet Plateau. In order to preserve Bos mutus genetic resource， three fibroblast cell strains from Bos mutus were established by tissue culture. Bos mutus cells in vitro culture appeard typical morphology of fibroblast cell and had strong hyperplasia ability. Population doubling time of the cells was 38.47 h， and plateau phase density of the cells was 2.08×106/mL. FGFR5 expression in cells was confirmed by immunofluorescence staining. Cell strains with FGFR5 positive expression were established by monoclonal cell culture. Chromosome karyotypes analysis to F7 cells showed that the rate of normal diploidy (2n=60) was 84.33%. Moreover， all autosomes were acrocentric chromosomes， and X as well as Y chromosome belonged to acrocentric chromosomes. Bos mutus cell strains eslablished in the study offered the material for the Bos mutus cloning research.
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