ANXA2 Regulates the Behaviors and Cytoskeletal Structure of caco2 Cells in Human Colorectal Cancer

Abstract: Annexin A2 (ANXA2) is an important member of Annexin family and it was confirmed to be abnormally expressed in various cancers and has important roles in cellular functions and fate. To further study the specific effects of ANXA2 on the behaviors and cytoskeletal structure exactly， the research was conducted to construct a ANXA2–/–caco2 cell line and to study the roles of ANXA2 playing in the biological behavior and cytoskeletal structure for caco2 cells. The results showed that the knockout of ANXA2 could significantly inhibit proliferation and migration in ANXA2–/–caco2 (P<0.05)， but it had no clear change on apoptosis. Depletion of ANXA2 in caco2 cells remarkably decreased the expression of F-actin and inhibited the development of pseudopodium and micro-villi， as evidenced by decreased proliferation and migration. Our data from the perspective of morphology objectively support that ANXA2 plays significant part in regulating the development of caco2 cells under the condition of target gene knockout， and the data also shows that ANXA2 may be a potential target for therapeutic strategies.

CSTR: 32200.14.cjcb.2013.12.0006