Study on Differences in Proteome and Transcriptome of Peripheral Blood Mononuclear Cells Induced by Cytokines

Abstract: We added Interferon-γ， anti-CD3 monoclonal antibody and IL-2 into culture medium in order to induce amplification of peripheral blood mononuclear cells into CIK cells， then collected cell samples before the induction culture and at 15 days of culture. The total proteins were extracted for cell counting， two-dimensional electrophoresis and silver staining as well as shape obversation and cell surface markers were detected before and after culture. Then， the expression of different protein spots were analyzed by ImageMasterTM software to analyze different proteins spots which were expressed the same or different before and after culture. There were 24 protein spots selected and mass spectrum identification. The representative proteins collected before and after culture were analyzed of their transcription by qPCR technology. The results showed that the proteomics characteristics of the cells before and after culture were completely different， for same expression of the protein spots， they mainly related to gene transcription factors and cytoskeleton. Meanwhile， specific proteins expressed after induction mainly relate to cell growth and proliferation. Though a negative correlation phenomenon is shown on the level of transcription and protein-binding， the proteome is the final form of the gene expression. Therefore， protein differences in results suggested that induced by the cytokines， heavy amplification of CIK cells was associated with its intracellular protein expression changes.

CSTR: 32200.14.cjcb.2013.12.0005