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Methods for Vital Fluorescent Labeling of Guard Cells Vacuole
Xin-Qi Gao*, Qiu-Ping Ren1, Zhuan-Bin Wang2
State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China; 1Agricultural College, Liaocheng University, Liaocheng 252029, China; 2College of Life Sciences, Qufu Normal University,
Abstract: Vital labeling of vacuole is required for the study of vacuolar dynamics. Several methods for vacuole labeling of guard cells in vivo were introduced in this paper by using of acridine orange， Lysotracker Red DND-99， BCECF-AM， fluorescein diacetate and GFP fusion protein. We found that the procedures of vacuolar labeling by acridine orange and fluorescein diacetate are simple and the results are better. In addition， GFP fusion protein might be the best strategy for vital vacuolar labeling in the future.
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