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Studies of Cytoskeleton in Oocytes and Preimplantation Embryos by Confocal Laser Scanning Microscopy
Ying Xu▲, Qing-Hua Zhang1▲, Na Guan1, Yan-Ning Xu1, Xiao-Fei Yan1, Lei Lei1*
Departpment of Anatomy and Histology, College of Medicine, Jiaxing University, Jiaxing 314001, China; 1Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China
Abstract: This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique. We observed the configuration， distribution and localization of β-tubulin， γ-tubulin， microfilaments and chromosomes in mouse oocytes and preimplantation embryos. We also described some key points and tricks of this technique， and discussed analysis method of the experiment results as well.
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