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Moderate Concentration of H2O2 Induced [Ca2+]i Oscillation in Human Bronchial Epithelial Cells
Yang Weibing1#, Wang Yongquan1#, Ye Qing2, Cai Pengcheng3, Zhu Liping4, Ye Hong4, Ma Wanli1*
1Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; 2Department of Computer Science and Technology, Huaihua University, Huaihua 418000, Chin
Abstract: Reactive oxygen species (ROS) including hydrogen peroxide (H2O2) can damage cells by inducing changes of cytosolic Ca2+ ([Ca2+]i). However， different concentration of H2O2 induced different changes in [Ca2+]i which activated different signaling pathways. One type of [Ca2+]i changing is [Ca2+]i oscillation which can regulate the activity of NF-κB. The aim of this study is to get the concentrations of H2O2 which can induce [Ca2+]i oscillation in
the bronchial epithelial cells. Human bronchial epithelial cells were cultured， and treated without or with different concentration of H2O2 (0~1 000 μmol/L). Cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2.
The results revealed that H2O2 concentrations <50 μmol/L just induced [Ca2+]i spark， 50~500 μmol/L induced [Ca2+]i oscillation， and 1 000 μmol/L induced constantly high level of [Ca2+]i. Moreover， the findings confirmed that 150 μmol/L H2O2 induced [Ca2+]i oscillation， and the [Ca2+]i oscillation induced increases of NF-κB activity. In conclusion， these data suggested that moderate concentration (50~500 μmol/L) of H2O2 can induce [Ca2+]i oscillation and NF-κB activation in bronchial epithelial cells， which should be involved in ROS induced damage in chronic airway inflammation.
the bronchial epithelial cells. Human bronchial epithelial cells were cultured， and treated without or with different concentration of H2O2 (0~1 000 μmol/L). Cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2.
The results revealed that H2O2 concentrations <50 μmol/L just induced [Ca2+]i spark， 50~500 μmol/L induced [Ca2+]i oscillation， and 1 000 μmol/L induced constantly high level of [Ca2+]i. Moreover， the findings confirmed that 150 μmol/L H2O2 induced [Ca2+]i oscillation， and the [Ca2+]i oscillation induced increases of NF-κB activity. In conclusion， these data suggested that moderate concentration (50~500 μmol/L) of H2O2 can induce [Ca2+]i oscillation and NF-κB activation in bronchial epithelial cells， which should be involved in ROS induced damage in chronic airway inflammation.
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