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Transfection of Genes into Cancer Cells Mediated by Cationic Liposomes
Zhao Yinan1,2, Zhang Shubiao1*, Cui Shaohui1, Zhang Chuanmin1, Zhang Shufen2
1Key Laboratory of Biotechnology and Bioresources Utilization, The State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian 116600, China; 2State Key Laboratory of Fine Chemicals, Dalian Universi
Abstract: The transfection of gene vectors into cancer cells is a key step for gene therapy. Lipofectamine 2000 and DOTAP are two commonly used transfection reagents as they have relatively higher transfection efficiency. The size， zeta potential and morphology of Lipofectamine 2000 and DOTAP were researched. Lipoplexes formed by the combination of cationic liposomes with pGFP-N2 were evaluated in terms of transfection efficiency and cytotoxicity by using two types of cells (Hep-2 and NCI-H460). The results indicated that both of Lipofectamine 2000 and DOTAP could condense DNA to form lipoplexes. Lipofectamine 2000 showed higher transfection efficiency compared to DOTAP with an optimal mass ratio range of 2/1 to 4/1. Lipofectamine 2000 and DOTAP showed cytotoxicityb to some degree under the optimal transfection conditions. Cell type was important influence factor for transfection， and Hep-2 cell was easier to be transfected than NCI-H460 with Lipofectamine 2000.
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