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The Cultivation and Proliferation of Rabbit's Amniotic Epithelial Cells in Vitro
Ling Jin, Jian Chen*, Jing Wu1, Qing Zhou, Xiao-Yong Liu, Fang Zhao, Jing-Tang Xu1, Song-Bin Zhao1
Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510632, China; 1Ophthalmology Laboratory, Medical College of Jinan University, Guangzhou 510632, China
Abstract: Rabbit's amniotic epithelial cells (AECs) were obtained from rabbits at the late trimester of pregnancy (27-28E) and culturated in vitro. The AECs were observed under the light microscope and the scanning electron microscope. By using immunohistochemical staining， the AECs were examined to evaluate the expression of cytokeratin with monoclonal antibody AE1/AE3 and AE5. The influence of EGF and fetal bovine serum (FBS) on the cell cycle of AECs was determined by using flow cytometry. Rabbits' AECs at the late trimester of pregnancy grew well and proliferated actively in vitro. Immunohistochemical staining showed that the expression of AE1/AE3 and AE5 of the cultured AECs was positive. Compared with the control group， the percentage of cells at different phrase changed. Those at the G0/G1 phase decreased， while cells at the S phase and G2/M phase increased. The cell proliferation index (PI) increased， P<0.01. The co-application of EGF and serum group was compared with the EGF group and FBS group respectively， P<0.05. The AECs had cytokeratin CK3. Both of EGF and FBS could promote the proliferation of AECs and the influence of the co-application of EGF and serum on the proliferation was more significant.
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