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The Expression of Calcium-activated Chloride Channel in PASMCs’ under Hypoxic Hypercapnia State and the Relationship with MAPK Pathways
Huang Linjing1#, Li Guanlong2#, He Jinbo1, Ma Yingchun1, Chen Dan1, Ying Lei1, WangYang1, Wang Wantie1*
1Department of Pathophysiology Wenzhou Medical University, Wenzhou 325035, China;2Zhejiang Provincial Academy of Medical Sciences and Health Research Institute of Toxicology Laboratory, Hangzhou 310013, China
Abstract: We adopt an enzyme digestion to take the male SD rat pulmonary artery smooth muscle cells (PASMCs) for cell primary culturing， taking immunofluorescence cytochemical method with mouse anti-rat the SM α-actin for cell identification. Then we set up a model of hypoxia and hypercapnia. Western blot is used to detect the protein expression of calcium-activated chloride channel (CLCA2); We Use semi-quantitative reverse transcriptionpolymerase chain reaction to detecte the mRNA expression of CLCA2. The results demonstrated that compared with normal group， the expressions of CLCA2 mRNA and protein in PASMCs were significantly raised in hypoxia hypercapnia group (P<0.01); Contrast to the hypoxic hypercapnic group， CLCA2 mRNA and protein expressions in PASMCs were significantly raised in group U and group SB (P<0.01); the mRNA and protein expression in PASMCs were significantly decreased in group A (P<0.05 and P<0.01). The expression of CLCA2 in PASMCs could be raised under hypoxia and hypercapnia conditions; The expression of CLCA2 in PASMCs could be raised by both U0126 and SB203580; The expression of CLCA2 in PASMCs could be decreased by Anisomycin.
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