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Effects of ABCE1 Gene Silence in Proliferation and Apoptosis of Human Breast Cancer Cells MCF-7
Gong Xiangnan1, Huang Bo1*, Zhou Hongli2
1Department of Thoracic Surgery, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, China;2Department of Kidney, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121000, China
Abstract: This paper aim at investigating the effects of ABCE1 gene silence in proliferation and apoptosis of human breast cancer cells MCF-7 and discuss its mechanism preliminarily. The siRNA vector of constructed ABCE1 was transfected into MCF-7. The efficiency of transfection was observed under the fluorescence microscope. Effects of gene silencing was confirmed by Western blot and RT-PCR. Expressions of RNase L protein was examined by Western blot. The proliferation was analysised by MTT and growth curve was drew. The apoptosis was analysised by flow cytometry. Results showed that ABCE1-siRNA was transfected into MCF-7 successfully. After transfection， cell viability reduced and expression of ABCE1 were blocked (P<0.01). The expression of RNase L increased obviously (P<0.01). The ability of cell proliferation decreased but cell apoptosis rate increased (P<0.01). From discussion we can draw the conclusion that ABCE1-siRNA can successfully silence ABCE1 gene in human breast cancer cells MCF-7. Silencing this gene can restrain the proliferation of human breast cancer cells MCF-7 and induce apoptosis.The mechanism may be related to blocking-up cell pathway of 2-5A/RNase L.
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