ZTO Inhibited Proliferation and Induced Apoptosis of Human Lung Adenocarcinoma Cell Line A549

Abstract: The study investigated the effects of zedoray turmeric oil (ZTO) on the proliferation and apoptosis of human lung adenocarcinoma cell line A549. The inhibitory effect of ZTO on the growth of A549 cells was tested by MTT method in vitro. Light microscope， fluorescence microscope and TEM were used to observe the morphology change of apoptosis in A549 cells. The apoptosis， mitochondrial membrane potential (ΔΨm) and cell cycle distribution of A549 cells were assayed by flow cytometry. The levels of Bcl-2/Bax were measured by Real Time RT-PCR and Western blot. The results showed that ZTO inhibited the growth of A549 cells in a dose-dependent manner， consistent with the results of cell morphology. After exposure to 80 μg/mL ZTO for 48 h， cell apoptosis was highest， the level of ΔΨm decreased and caused a significant S arrest at the expense of G1 and G2-M phase cell numbers. ZTO also reduced the ratio of Bcl-2/Bax. The results suggested that ZTO depresses the cell growth and induces the apoptosis of human lung adenocarcinoma cell line A549 through up-regulation of Bax and down-regulation of Bc1-2.

CSTR: 32200.14.cjcb.2013.09.0007