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Adipose-derived Mesenchymal Stem Cells Promoted the Proliferation and Invasion of Pancreatic Cancer Cells by SDF-1 in Vitro
Yu Fuxiang1, Song Caixin1, Wu Zhiwei1, Zhu Qiandong1, Ji Shiqiang2, Zhang Qiyu1*
1Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Wenzhou Medical College,Wenzhou 325000, China;2Department of Liver Surgery І, Eastern Hepatobiliary Surgery Hospital,Second Military Medical Un
Abstract: The aim was to explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer (PaCa) cells in vitro and the the possible mechanism involved. ADSCs were isolated and co-cultured with PaCa cells. CCK-8 assay was used to detect the proliferation of PaCa cells. An ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of SDF-1 and CXCR4 in PaCa cells and ADSCs. The proliferation of PaCa cells by SDF-1 was measured. AMD3100 regulated the activity of PaCa cells after co-culture of ADSCs and PaCa.ADSCs could promote the proliferation and invasion of PaCa cells; The expression of SDF-1 was high in ADSCs，but not in PaCa cells. On the contrary， Higher CXCR4 mRNA levels were detected in the PaCa cells compared to ADSCs. The promotion of SDF-1 on PaCa cells depended on the concentration of SDF-1; AMD3100 significantly downregulated these growth-promoting effects of ADSCs on PaCa cells. ADSCs could promote the roliferation and invasion of PaCa cells， which might involve the SDF-1/CXCR4 axis.
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