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Effect and Mechanism of Lumican Gene Overexpression on Metastasis of Lung Adenocarcinoma Cell A549
Zhou Kun1, Wang Geng1, Pan Junfeng1, Ge Mingjian2*
1Department of Endocrine and Vascular Surgery, Affiliated Taihe Hospital of Hubei University of Medicine, Shiyan 442000, China;2Department of Thoracocardiac surgery, the First Affiliated Hospital, Chongqing Medical University, Chongq
Abstract: Lumican which widely distributed in human tissues and abnormally expressed in a variety of malignant tissues played an important role in tumor migration， adhesion and metastasis formation process. Firstly， the stable overexpression of Lumican in lung adenocarcinoma cell line A549 was established in the study. Furthermore， transwell chambers assay， adhesion， angiogenesis and animal experiments were used to detect the ability of invasion， migration， angiogenesis， homogeneous and heterogeneous cells intercellular adhesion among each group， and to investigate the effect of the metastasis on A549. Finally，Western blot was used to detect the expression of RhoC， p-Akt， MMP-2 and VEGF proteins in order to illustate the possible mechanism. Compared with the control group and (or) the empty vector group， the results showed that the experimental group cells invasion and migration ability were enhanced (P<0.05) and homogeneous cells intercellular adhesion were decreased. Meanwhile， heterogeneous cells intercellular adhesion was incresed (P<0.05); Angiogenesis capacity and the vascular density which was got from nude mice subcutaneous tumor were increased (P<0.05); The expression of RhoC， p-Akt， MMP-2 and VEGF were significantly rised (P<0.05) and there were no differences between the control group and empty vector group (P>0.05). The study indicated that the overexpression of Lumican gene could promote the metastasis of lung adenocarcinoma cell A549 in vitro， and its mechanism might be related to the increased expression of the RhoC protein.
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