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Gene Silencing on hnRNPA2B1 Promotes Apoptosis of Renal Cell Carcinoma Cells
Yang Shilai1, Chen Yong2, Zhang Weili1*, Zhang Jianhua2*
1Department of Urology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 40001,china;2Department of Urology, Fuling Central Hospital, Chongqing 408000, China
Abstract: To investigate the effects of hnRNPA2B1 gene silencing on apoptosis of 786-0 cells and its potential mechanism， hnRNPA2B1 shRNA recombinant plasmids were constructed and transfected into renal cell carcinoma 786-0 cells. The effects of hnRNPA2B1 shRNA recombinant plasmids on hnRNPA2B1 gene silencing and the expression of Bcl-X isoforms were detected by RT-PCR. The apoptosis of 786-0 cells were assayed by flow cytometry. MTT method was used to observe the proliferation ability of 786-0 cells. Compared to the control groups， the apoptosis rates of gene-silencing groups were increased significantly while the cell multiplication rates were notably decreased (P<0.05); The mRNA expression levels of pro-apoptotic factor Bcl-X(S) and the ratio of Bcl-X(S)/Bcl-X(L) were increased significantly after the hnRNPA2B1 gene have been silenced (P<0.05). In conclusion， hnRNPA2B1 gene silencing could effectively promote apoptosis of 786-0 cells， the mechanism of which probably relates to its affection on the expression of Bcl-X(S).
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