Home > Browse Issues > Vol.35 No.8
Molecular “Scissors” —TALENs Mediated Site-specific Gene Modification Technology
He Lixiazi1, Chen Haide2, Xiao Lei2*
1Shanghai Institute for Biological Sciences, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences,Shanghai 200031, China;2College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Abstract: Transcription activator-like effectors nucleases (TALENs)， has been a new important genetic tools for targeted gene modification these years. TALENs are constructed to mediate multiple genetic manipulation including gene targeting， gene site-specific insertion or correction， through forming double-strand break (DSB). The advantage of this technology is that TALENs mediate site-specific modification much more easily compared to the former methods: each repeat of TALENs recognizes one base of target DNA， which makes the designation and construction of TALENs more efficiently， and TALENs are able to manipulate the genomes of different species without epigenetic gene insertion and manipulate genome in both cellular and organismal level. Here， we review the recent progress and prospects of TALENs technology.
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