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Expression of Oct4 in Breast Cancer Tissue and Its Effect on Cell Invasion of Breast Cancer
Cao Yong1, Zhang Yunyun2, Xie Jia1, Zeng Xiaohua3, Wu Chengyi1*
1Department of Endocrine Surgeons, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China;2Department of Oncology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China;
Abstract: This paper examined the expression level of Oct4 in breast cancer tissue and further investigate its effect on cell invasion of breast cancer. Western blot analysis was used to detect the protein level of Oct4 in 27 paired breast cancer tissue and adjacent non-tumoral breast tissue. Oct4 expression in breast cancer cells was knockdown by lentivirus-mediated shRNA interference technology. The effect of oct4 silencing on the cell invasion was determined by wound-healing assay and cell invasion assay. Four EMT markers (E-cadhrin， alpha-catenin， vimentin and N-cadherin) were analyzed by Western blot analysis. The results demonstrated that 74% (20/27) patients
showed elevated Oct4 expression in breast cancer tissue compared with that in adjacent non-tumoral breast tissue.Lentivirus-mediated shRNA significantly suppressed Oct4 expression. Gene silencing of oct4 markedly inhibited invasion of breast cancer cells. Knockdown of oct4 in breast cancer cells induced the expression of epithelial markers E-cadherin and α-catenin that was accompanied by a concomitant reduction of mesenchymal marker N-cadherin and vimentin. These finding suggested that Oct4 expression was upregualted in breast cancer tissue. Gene silencing of oct4 inhibited cell invasion of breast cancer through regulating EMT.
showed elevated Oct4 expression in breast cancer tissue compared with that in adjacent non-tumoral breast tissue.Lentivirus-mediated shRNA significantly suppressed Oct4 expression. Gene silencing of oct4 markedly inhibited invasion of breast cancer cells. Knockdown of oct4 in breast cancer cells induced the expression of epithelial markers E-cadherin and α-catenin that was accompanied by a concomitant reduction of mesenchymal marker N-cadherin and vimentin. These finding suggested that Oct4 expression was upregualted in breast cancer tissue. Gene silencing of oct4 inhibited cell invasion of breast cancer through regulating EMT.
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