Establishment of Human Fibroblast and Trophoblast Cell Lines with Abnormal Karyotypes

Abstract: To establish human fibroblast and trophoblast cell lines by using abnormal karyotypes of cells isolated from the fetus amniotic fluid and chorionic villus. Fibroblast and trophoblast cells were obtained from fetus amniotic fluid or chorionic villus that was used for chromosome analysis. Only cells with abnormal karyotypes identified by G band were further cultured. Cell types in each cell line were identified by immunocytochemistry， PowerPlex 16 System Kit and DNA-STR. As results， a 21 trisome cell line from amniotic fluid and 7 trophoblast cell lines from chorionic villus were established. The karyotypes of these cell lines were 47， XX+21; 69， XXX; 69， XXY; 47， XY+12; 47， XX+5; 48， XY+21， 22 and 47， XY+18， respectively. All cell lines have undergone more than 10 passages and more than 50% of the cells were survival after freezing/thawing. The karyotypes remained stable during the culture. These results indicate that fibroblast and trophoblast cell lines with abnormal karyotypes can be established in human and these cell lines are important cell sources for studying of the basic mechanism of chromosome abnormalities and spontaneous abortion.

CSTR: 32200.14.cjcb.2008.03.0020