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Analysis of Aberrantly Expressed MiRNAs in Intermittent High Glucose Induced Renal Epithelial-Messenchymal Transition
Cao Luoyuan, Huang Baoying*, Fu Xianguo, Yang Jing, Lin Feng
The Affiliated Ningde Municipal Hospital of Fujian Medical University, Ningde 352100, China
Abstract: The miRCURYTM microRNA chip (v.16.0) was used to evaluate miRNA expression levels between intermittent high glucose-induced renal epithelial-messenchymal-transition (EMT) cells and normal group cells; an average change more than 2-fold (P<0.05) was set as a standard variance level. The results showed that 49 miRNAs were upregulated and 6 miRNAs were reported in renal EMT; 34 miRNAs were downregulated in intermittent high glucose-induced renal EMT cells and 8 miRNAs were reported in renal EMT. RT-qPCR was used to verify the miRCURYTM microarray results and the Pearson correlation of relative miRNAs expression levels was analyzed by miRCURYTM microarray vs RT-qPCR (r=0.98， P<0.01). The results of miRCURYTM microarray are accordant with those of RT-qPCR， which indicates that miRNAs may play a role in intermittent high glucose-induced renal EMT process. These results provide a novel therapy and early diagnosis target against renal EMT process.
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