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MoTCTP， A Homolog of Translationally Controlled Tumor Protein， Is Required for Fungal Growth and Conidiation in Magnaporthe oryzae
Zhang Lilin1, Cao Huijuan2, Li Xiaodong1, Lin Fucheng2,3, Feng Xiaoxiao2, Lu Jianping1*
1School of Life Science, Zhejiang University, Hangzhou 310058, China;2Biotechnology Institute, Zhejiang University, Hangzhou 310058, China;3China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC,
Abstract: Translationally controlled tumor protein (TCTP) is a multifunctional protein abundantly expressed in multicellular organisms. It plays important roles in lots of biological processes， such as cell growth，differentiation and gene expression in animal and plant. In this report， we described the expression， distribution，and function of MoTCTP， a homolog of human TCTP， in the rice blast fungus Magnaporthe oryzae. MoTCTP is highly expressed in hyphae， conidia and appressoria. MoTCTP is localized to cytoplasm， mitochondrial outer membrane and cytoplasm nearby vacuoles. Knockout of MoTCTP led to reduction in mycelial growth， decrease in conidial production and delay in conidial germination in the fungus， but increase in resistance to hydrogen peroxide stress. The expression of six genes encoding cyclins and cell division control proteins and three genes encoding known conidiogenesis related proteins were downregulated in MoTCTP null mutants， but three genes encoding cyclin-dependent kinases were upregulated among fifteen analyzed genes. These results demonstrated the biological function of MoTCTP in the control of fungal development and in the response to cellular stresses in rice blast fungus and suggested that cell cycle regulation proteins might be involved in these process regulated by MoTCTP.
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