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RNAi-mediated PRR11 Depletion Influences Cell Cycle Regulation in Lung Cancer H1299 Cells
Zhang Chundong1,2, Wang Yitao1,2, Zhang Ying1,2, Li Yi1,2, Zhu Huifang1,2, Cai Wei1,2,Zhu Yuanyuan1,2, Bu Youquan1,2*
1Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China;2Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China
Abstract: Proline-rich protein 11(PRR11) is a novel tumor associated gene， implicated in both cell cycle progression and lung cancer. Studies have showed that downregulation of PRR11 by siRNA resulted in cell cycle arrest at S phase in cancer cells. In the present study， we analyzed the molecular mechanism of downregulation of PRR11 caused cell cycle arrest in lung cancer cell with FCM analysis and IF staining. Brdu labelling showed that downregulation of PRR11 caused cell cycle S phase arrest， and the downregualtion of ribonucleotide reductase resulted in cell cycle arrest by adding dNTPs in vitro. Mitotic marker pHH3 labelling showed silencing PRR11 caused cell cycle arrest at G2 phase leading to inhibition in mitosis. Abnormal expression of PRR11 may result in dysregulation of cell cycle progression and promote tumorigenesis.
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