Home > Browse Issues > Vol.35 No.8
Ultrastructural Observation of Fertile and Sterile Pollen Grains of A Thermo-sensitive Genic Male-sterile Wheat
Li Dongxiao1, Deng Xiaoli2, Feng Suwei1, Xu Longlong1, Ru Zhengang1*
1Henan Institute of Science and Technology, Xinxiang 453003, China;2Department of Life Sciences and Technology,Xinxiang University, Xinxiang 453003, China)
Abstract: The fertile and sterile pollen grains of thermo-sensitive male sterile wheat (Triticum aestivum L.) were observed using electron microscope to find the structural feature of sterile pollen grains. Before microspore division， the structure of microspore of sterile line was normal. The earliest abnormal phenomenon in sterile pollen grains appeared in bicellular pollen grains: in fertile line， after microspore divided to form bicellular pollen grains，the big vacuole in the vegetative cell became small size by forming some small vacuoles with single member.Then the big vacuole filled some content and became cytoplasm matrix. Following the big vacuole decomposing in vegetative cell， some starches grains accumulated in the pollen. In sterile pollen grains， microspore could divide to form a bicellular pollen. The vegetative cell， however， formed some autophagosomes moving into big vacuole，which resulted in the big vacuole of vegetative cell not decomposing but enlarging. The bicellular pollen kept the big vacuole all the time. Then， the pollen cytoplasm decreased and pollen aborted. The determination of abortion time and abortion structure of this male sterile wheat makes a foundation for further more researching its abortion mechanism.
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