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Effect of Overexpressed S100A6 on Osteogenic Differetation of BMP9 Induced Mesenchynal Stem Cell C3H10T1/2
Ye Liwei, Wu Rui, Duan Liang, Zhang Yunyuan, Yang Xia, Chen Xian, Wang Haiyan, He Tongchuan, Zhou Lan*
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the effects of S100A6 in BMP9-induced osteogenic differentiation of mesenchymal stem cell line C3H10T1/2， C3H10T1/2 cells were co-infected with the recombinant adenovirus AdBMP9 and AdS100A6.Immunocytochemistry， alkaline phosphatase staining， activity analysis， and alizarin red S staining were used to detect the markers of osteogenic differetation， including Runx2， alkaline phosphatase (ALP)， osteopontin (OPN)， and calcium deposition; Western blot was used to measure the β-catenin expression. S100A6 had nether significant effects on Runx2 protein levels at 3 d nor ALP at 7 d， and the same to calcium deposition at 18 d (P>0.05). But， it increased OPN at 12 d (P<0.05). Meanwhile， S100A6 decreased the β-catenin level at 12 d (P<0.05)， but it didn’t change the level of β-catenin at 3 d and 7 d (P>0.05). S100A6 over-expression can promote the OPN expression and decrease the β-catenin level in BMP9-induced C3H10T1/2 cells， but it has no significant effect on the final osteogenic differentiation.
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