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Expression of Calreticulin in the Endometrium of Early Pregnant Mouse
Yan-Ling Dong, Jun-Lin He, Xue-Qing Liu, Yu-Bin Ding, De-Pei Huang, Ying-Xiong Wang*
Laboratory of Reproductive Biology, Department of Genetics, School of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: To probe the role of calreticulin (CRT) in blastocyst implantation， we have detected expression of CRT mRNA and the protein in endometria from mice of non-pregnancy and pregnancy on days 1， 2， 3， 4， 5， 6， 7， respectively by RT-PCR， indirect immunofluorescence histochemistry， Western blot and in situ hybridization techniques. The results showed that the expression of CRT mRNA and the protein is higher in pregnant mice than that of non-pregnant mice， and reach maximal level pregnant on day 4， 5 and was located in the uterine stromal cells and epithelium. The result suggested that the expression characteristic of CRT might involved in adhesion/invasion of the blastocyst to endometrium and be useful for embryo implantation and maintenance of pregnancy in mouse.
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